Total Glutathione (T-GSH)/Oxidized Glutathione (GSSG) Colorimetric Assay Kit (E-BC-K097-S)
For research use only.
| Detection Principle | GSSG is reduced to GSH by glutathione reductase, and GSH can react with DTNB to produce GSSG and yellow TNB. The amount of total glutathione (GSSG+GSH) determines the amount of yellow TNB. Thus the total glutathione can be calculated by measuring the OD value at 412 nm. The content of GSSG can be determined by first removing GSH from the sample with appropriate reagent and then using the above reaction principle. |
| Synonyms | GSSG;T-GSH |
| Sample Type | Serum,Plasma,Animal tissue,Red blood Cell,cultured Cell |
| Detection Method | Colorimetric method |
| Detection Instrument | Spectrophotometer (412 nm) |
| Research Area | Oxidative Stress |
| Other Reagents Required | PBS (0.01 mol/L, pH 7.4), Absolute ethanol |
| Storage | This product can be stored at 2-8°C/-20°C for 12 months with shading light. Please refer to the manual for the specific storage condition of the components. |
| Valid Period | 12 months |
| Sensitivity | 0.12 μmol/L T-GSH |
| Detection Range | 0.12-30 μmol/L T-GSH |
| Precision | inter-assay CV: 4.7% | intra-assay CV: 0.9% |
| Sample Volume | 100 μL |
| Assay Time | 3 h |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| Human serum | 1 |
| Rat plasma | 1 |
| Mouse serum | 1 |
| 10% Rat heart tissue homogenate | 10 |
| 10% Rat liver tissue homogenate | 60 |
| 10% Rat brain tissue homogenate | 10 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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