Total Antioxidant Capacity (T-AOC) Colorimetric Assay Kit (ABTS, Chemical Method) (E-BC-K271-M)
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For research use only.
| Detection Principle | The principle of the ABTS method for determining the T-AOC is as follows. ABTS is oxidized to green ABTS+ by appropriate oxidant, which can be inhibited if there exist antioxidants. The T-AOC of the sample can be determined and calculated by measuring the absorbance of ABTS+ at 734 nm. Trolox is an analog of VE and has a similar antioxidant capacity to that of VE. Trolox is used as a reference for other antioxidant antioxidants. For example, the T-AOC of Trolox is 1, then the antioxidant capacity of the other substance with the same concentration is showed by the ratio of its antioxidant capacity to Trolox antioxidant capacity. |
| Synonyms | T-AOC |
| Sample Type | Serum,Plasma,Urine,Saliva,Tissue,Cell |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (730-740 nm) |
| Research Area | Oxidative Stress, Plant Physiology Series |
| Other Reagents Required | Double distilled water, 1 × PBS, 80% Ethanol |
| Storage | This product can be stored at -20°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 0.05 mmol/L |
| Detection Range | 0.05-1.00 mmol/L |
| Precision | inter-assay CV: 0.05 | intra-assay CV: 4.1% |
| Sample Volume | 10 μL |
| Assay Time | 20 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| 20% Tomato tissue homogenization | 2-5 |
| 10% Mouse heart tissue homogenization | 8-12 |
| 10% Mouse liver tissue homogenization | 8-12 |
| 10% Mouse lung tissue homogenization | 8-12 |
| Human saliva | 2-5 |
| Human urine | 15-30 |
| Human serum | 15-30 |
| Human plasma | 8-15 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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