Total Amino Acids (T-AA) Colorimetric Assay Kit (E-BC-K055-S)
For research use only.
| Detection Principle | Copper ions can form blue-green complexes with various amino acids. The intensity of the color of the complex at 650 nm is directly proportional to the total content of amino acids. |
| Sample Type | Serum,Plasma,Animal and plant tissue,Urine |
| Detection Method | Colorimetric method |
| Detection Instrument | Spectrophotometer(635-655 nm, optimum wavelength: 650 nm) |
| Research Area | Amino Acids And Proteins |
| Other Reagents Required | PBS(0.01 M，pH 7.4) |
| Storage | This product can be stored at 2-8°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 4.73 mmol/L |
| Detection Range | 4.73-100 mmol/L |
| Precision | inter-assay CV: 3.5-6.3% | intra-assay CV: 3.1-4.9% |
| Sample Volume | 80 μL |
| Assay Time | 1 h 10 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| Human serum | 1 |
| Human urine | 1 |
| Rat serum | 1 |
| 10% Rat lung tissue homogenate | 1 |
| 10% Rat spleen tissue homogenate | 1 |
| 10% Rat liver tissue homogenate | 1 |
| 10% Mouse kidney tissue homogenate | 1 |
| 10% Epipremnum aureum leaf tissue homogenate | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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