TNF RI/TNFRSF1A Polyclonal Antibody (AN005930L)
For research use only.
| Verified Samples | Verified Samples in WB: HeLa, A549, C6 |
| Dilution | WB 1:500-1:1000 |
| Isotype | IgG |
| Host | Rabbit |
| Reactivity | Human, Rat |
| Applications | WB |
| Clonality | Polyclonal |
| Immunogen | Recombinant Mouse TNF RI/TNFRSF1A protein expressed by E.coli |
| Abbre | TNF RI/TNFRSF1A |
| Synonyms | CD120a, FPF, MS5, p55-R, p60, TBP1, TNFAR, TNFR1, TNFR1-d2, TNFR55, TNF-R55, TNFR60, TNF-R-I, TBP, TNFalpha-R, TNF-alphaR, TNFRp, TNFR1-d, FPF, MS5, p55, p55-R, p60, TBP1, TNFalpha-R1, TNF-alphaR1, TNFAR, Tnfr, TNF-R, TNF-R1, TNFR1-d2, TNFR55, TNF-R55, TNFR60, TNFRI, TNF-R-I, TNFRp55, Tnfr-1, Tnfr1, Tnfrsf1a, CD120a, TNFR-I, p55, TNFRSF1a |
| Swissprot | |
| Calculated MW | 50 kDa |
| Observed MW |
50 kDa
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Cell membrane, Golgi apparatus membrane |
| Concentration | 1 mg/mL |
| Buffer | PBS with 0.05% proclin 300, 1% protective protein and 50% glycerol,pH7.4 |
| Purification Method | Antigen Affinity Purification |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | The product is shipped with ice pack, upon receipt, store it immediately at the temperature recommended. |
| background | TNF-α is an important cytokine produced by numerous cell types, including neutrophils, activated lymphocytes, macrophages, and NK cells. It plays a critical role in inflammatory responses and apoptosis. TNF-α exists as a membrane-anchored and soluble form, both of which show biological activity. Response to TNF-α is mediated through two receptors, TNF-R1, which is widely expressed, and TNF-R2, which is expressed mainly in immune and endothelial cells. Antagonists to TNF-α have been validated as therapeutic targets for rheumatoid arthritis and other immune disorders. The two receptors for TNF-α, TNF-R1 (55 kDa) and TNF-R2 (75 kDa) can mediate distinct cellular responses. In most cases cytotoxicity elicited by TNF has been reported to act through TNF-R1. Cytotoxicity is mediated by a "death domain" with the intracellular region of the receptor that binds to the death domain adaptor protein TRADD and triggers the activation of caspases (8). Soluble forms of both receptors have also been characterized which can bind TNF-α and may play an important role in immune disorders. |
Other Clones
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Unconjugated
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