For research use only.
| Product Introduction | TM4 Cell Complete Medium is a ready-to-use medium that can be directly used for the culture of TM4 cells, which is carefully designed and optimized by Pricella's R&D team based on DMEM/F12 basal medium. This high-quality complete medium has been strictly verified by TM4 cells which could promote cell proliferation and maintain cell properties. |
| Concentration | 1× |
| Format | Liquid |
| Mycoplasmal Detection | Negative |
| pH | 7.4-7.8 |
| Green Features | Sustainable packaging |
| Endotoxin Level | ＜3 |
| Storage | This product can be stored at 2-8℃ for 3 months with shading light. |
| Shipping | Ice bag |
| Expiration Date | 3 months |
| Matters Need Attention | 1. This product is for research use only. 2. This product is sterilized by 0.1 μm filtration. 3. It is necessary to pay attention to the aseptic operation and avoid the contamination during the culture. 4. It is not suitable for long time storage at room temperature. 5. This product is a ready-to-use medium. If there is no special need, don't add serum, penicillin and streptomycin. It can be used directly. |
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Q1:Will improper storage of my culture reagents affect the growth rate of my culture?
Yes. Store animal sera at -5 to -20℃. Store media at 2 to 8℃; use within recommended shelf life period. Store complete media (supplemented) at 2 to 8℃, and for complete medium the recommended shelf life is 3 to 6 months. Additionally, minimize exposure of sera and media to light.
Q2:Where can I find the osmolality for Medium?
The osmolality is listed in the COA for the particular lot number of the medium.
Q3:What factors can contribute to rapid cell death/culture failure?
There are a number of events that can contribute to this: 1. Incorrect CO2 levels:Monitor the level of CO2 manually with a Fyrite kit, available from Bacharach. Check if the manual readings concur with the readings displayed on the incubator. If the incubator has a trace readout, check the printout for fluctuations in CO2 level. Check the settings to ensure that CO2 levels are set at appropriate levels for your cell line (usually between 5 and 10%). Check line connections frequently for leaks. Avoid frequent opening and closing of incubator doors. 2. Temperature fluctuations in the incubator:Monitor the temperature of incubator with a good thermometer inside the incubator. 3. Amphotericin B or other preventive antibiotics/antimycotics are present at toxic concentrations:Use at recommended levels. 4. Humidity is incorrect:Check the water level in the water pan. Humidity is vital to appropriate gas exchange for many types of cells and media. 5. Incorrect osmotic pressure in medium:Check osmolality of complete medium. Most mammalian cells can tolerate an osmolality of 260 to 350 mOsm/kg. Additions of reagents such as HEPES and drugs may affect osmolality. 6. Contamination by microorganisms:Bacterial and fungal contaminations are usually easily visible; symptoms of mycoplasma contamination are more subtle, and careful monitoring of culture morphology and regular testing are necessary to detect this type of contamination. 7. Inappropriate medium is being used:Double-check that the medium used is appropriate for your cell type and culture application. For example, ensure that the medium being used for serum-free culture is actually designed for serum-free culture; make sure that appropriate selective drugs are used at appropriate levels; check the expiration dates for the reagents being used; and store medium at appropriate temperatures in the dark.
Q4:My medium was shipped at room temperature but it is supposed to be stored refrigerated. Is it okay?
We routinely ship media that require long-term storage in the refrigerator at room temperature. We have done studies on representative media formulations to show that media can be at room temperature for up to a week without a problem.
Q5:My cells are growing very slowly. What could be the cause of this?
Please see some common reasons below, and solutions to the issue: - Growth medium is not correct: Use pre-warmed growth medium as recommended by the supplier. - Serum in the growth medium is of poor quality: Use serum from a different lot. and choose good quality serum to ensure nutrition. - Cells have been passaged too many times: Use healthy, low-passage number cells. - Cells were allowed to grow beyond confluency: Passage mammalian cells when they are in the log-phase before they reach confluency. - Culture is contaminated with mycoplasma: Discard cells, media, and reagents. Obtain new stocks of cells, and use them with fresh media and reagents.
Q6:I see a precipitate in my medium, and a change in the pH. What should I do?
This is most likely a sign of bacterial or fungal contamination. We would recommend discarding the media, and trying to decontaminate the culture.
Q7:How long can I keep my media after supplementing with serum?
Generally speaking, media can be used for up to 3-6 months after supplementation with serum， at 2 to 8℃ low temperature environment preservation.
Q8:How light sensitive is media? Should I also be protecting it from LED light?
While we know that different wavelengths of light are worse than others for exposure, we would recommend as a best practice to protect the medium from all forms of light exposure including LEDs, as much as possible to ensure optimal performance, as several components within the medium are light sensitive, such as vitamins.When placed in a light-proof 4°C refrigerator with the door closed, the culture medium will be kept away from light.
Q9:Do you provide customized culture media services?
Yes. We can customize culture media with reduced components or changes in the concentration of existing components.
