Thiobarbituric Acid Reactants (TBARS) Fluorometric Assay Kit (E-BC-K298-F)
For research use only.
| Detection Principle | TBARS and TBA can react under high temperature and acid conditions and then form a pink compound, the concentration of which is linearly related to the concentration of TBARS in the sample. The TBARS concentration can be calculated by measuring the fluorescence values at the excitation wavelength of 530 nm and the emission wavelength of 550 nm. |
| Synonyms | TBARS |
| Sample Type | Serum,Plasma,Animal tissue,Cell |
| Detection Method | Fluorometric method |
| Detection Instrument | Fluorescence microplate reader (Ex/Em=520 nm/550 nm) |
| Research Area | Oxidative Stress |
| Other Reagents Required | Acetic acid, Normal saline (0.9% NaCl), PBS (0.01 mol/L, pH 7.4) |
| Storage | This product can be stored at 2-8°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 0.09 μmol/L |
| Detection Range | 0.09-10 μmol/L |
| Precision | inter-assay CV: 2.8% | intra-assay CV: 1.7% |
| Sample Volume | 0.1 mL |
| Assay Time | 1 h 20 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| Human serum | 1 |
| Rat serum | 1 |
| 10% Mouse brain tissue homogenization | 1 |
| Human plasma | 1 |
| Mouse serum | 1 |
| 10% Rat liver tissue homogenization | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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