Tartrate Resistant Acid Phosphatase (TRAP) Activity Assay Kit (E-BC-K871-M)
For research use only.
| Detection Principle | The chromogenic agent can be catalyzed by acid phosphatase to produce p-nitrophenol in acidic condition. P-nitrophenol has a maximum absorption at 405 nm. The activity of TRAP can be calculated by measuring the amount of produced p-nitrophenol. The activity of acid phosphatase being detected in the presence of tartric acid is regarded as TRAP activity. |
| Synonyms | TRAP |
| Sample Type | Serum,Plasma,Urine,Animal tissue,Cell sample |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (410-415 nm, optimum wavelength: 405 nm) |
| Research Area | Metabolic Diseases |
| Other Reagents Required | Normal saline (0.9% NaCl) |
| Storage | This product can be stored at -20°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 0.911 U/L |
| Detection Range | 0.911-100 U/L |
| Precision | inter-assay CV: 2.4-3.2% | intra-assay CV: 1.6-2.5% |
| Sample Volume | 40 μL |
| Assay Time | 25 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| Goat plasma | 1 |
| Human urine | 1 |
| Porcine serum | 1 |
| Rat plasma | 1-2 |
| 10% Mouse liver tissue homogenate | 5-8 |
| 10% Mouse kidney tissue homogenate | 3-5 |
| 10% Mouse lung tissue homogenate | 2-5 |
| 10% Mouse brain tissue homogenate | 3-5 |
| 1 × 10^6 HL-60 cells | 1 |
| 1 × 10^6 293T cells | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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