TAGLN Polyclonal Antibody (E-AB-70234)
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For research use only.
| Verified Samples |
Verified Samples in WB: Mouse stomach, Mouse colon, Rat stomach, Rat colon Verified Samples in IHC: Human lung cancer, Human breast cancer, Mouse spleen, Rat kidney |
| Dilution | WB 1:500-1:2000, IHC 1:300-1:800 |
| Isotype | IgG |
| Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC |
| Clonality | Polyclonal |
| Immunogen | KLH conjugated Synthetic peptide corresponding to Mouse SM22α |
| Abbre | TAGLN |
| Synonyms | 22 kDa actin binding protein, 22 kDa actin-binding protein, Human 22kDa smooth muscle protein, Protein WS3-10, SM22, SM22 alpha, SM22-alpha, SMCC, Smooth muscle protein 22-alpha, TAGL, TAGLN, TAGLN1, Transgelin, Transgelin variant 2, WS3 10 |
| Swissprot | |
| Calculated MW | 22 kDa |
| Observed MW |
22 kDa
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Cytoplasm |
| Concentration | 0.8 mg/mL |
| Buffer | Phosphate buffered solution, pH 7.4, containing 0.05% stabilizer, 1% protein protectant and 50% glycerol. |
| Purification Method | Affinity purification |
| Research Areas | Cancer, Cardiovascular, Signal Transduction |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended. |
| background | The transgelin family is a group of proteins that belong to 22kd actin-related corpnin superfamily. Of all three isoforms,transgelin 1 is the best characterized. Transgelin 1,also known as SM22alpha,is a specific marker for differentiated smooth muscle cells. Transgelin 2,also known as SM22 beta,is expressed by both smooth muscle and non-smooth muscle cells in a temporally and spatially regulated pattern. Trangenlin 3,also known as NP25,is only found in highly differentiated neuronal cells. |
Other Clones
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Unconjugated
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