Sucrose Synthase-Ⅰ (SS-Ⅰ) Activity Colorimetric Assay Kit (E-BC-K1208-M)
For research use only.
| Detection Principle | Sucrose synthase-I (SS- I) catalyzes the substrate reaction to generate reducing sugar. The reducing sugar can show color changes when reacting with the chromogenic agent. There is a characteristic absorption peak at 540 nm. The activity of the enzyme is directly proportional to the absorbance. |
| Sample Type | Plant tissue |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (530-550 nm, optimum wavelength: 540 nm) |
| Research Area | Plant Physiology Series |
| Storage | This product can be stored at -20°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 0.39 U/mL |
| Detection Range | 0.39-66.67 U/mL |
| Precision | inter-assay CV: 5.1-9.2% | intra-assay CV: 3.7-5.4% |
| Sample Volume | 40 μL (tissue homogenate) |
| Assay Time | 1 h 20 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| 10% Potato tissue homogenate | 1 |
| 10% Green peppers tissue homogenate | 1 |
| 10% Chinese cabbage leaf tissue homogenate | 1 |
| 10% Corn seed tissue homogenate | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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