Sucrose Synthase-Ⅱ (SS-Ⅱ) Activity Colorimetric Assay Kit (E-BC-K1207-M)
For research use only.
| Detection Principle | Sucrose synthase-Ⅱ (SS-Ⅱ) catalyzes the reaction of substrate to produce sucrose, which reacts with chromogenic agents to produce chromogenic substances. The characteristic absorption peak at 480 nm is found, and the enzyme activity is proportional to the color. |
| Sample Type | Plant tissue |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (470-490 nm, optimum wavelength: 480 nm) |
| Research Area | Plant Physiology Series |
| Storage | This product can be stored at -20°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 30 U/g wet weight |
| Detection Range | 110-4000 U/g wet weight |
| Precision | inter-assay CV: 2.0-3.5% | intra-assay CV: 1.5-2.0% |
| Sample Volume | 80 μL(tissue homogenate) |
| Assay Time | 50 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| 5% Corn seed tissue homogenate | 1-5 |
| 5% Potato tissue homogenate | 1 |
| 5% Sweet potato tissue homogenate | 1-5 |
| 5% Green dill leaf tissue homogenate | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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