Sucrase Activity Assay Kit (E-BC-K751-M)
For research use only.
| Detection Principle | Sucrase catalyzes its substrate (sucrose) to produce glucose, which produces hydrogen peroxide under the action of glucose oxidase. Hydrogen peroxide reacts with chromogenic agent to produce red substance, which has a strong absorption peak at 505 nm. In a certain concentration range, It's absorbance is proportional to glucose concentration. Therefore, the activity of sucrase can be calculated by measuring the OD value at 505 nm. |
| Sample Type | Animal tissue |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (500-520 nm, optimum wavelength: 505 nm) |
| Research Area | Plant Physiology Series |
| Other Reagents Required | PBS (0.01 mol/L, pH 7.4) |
| Storage | This product can be stored at 2-8°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 20 U/mL |
| Detection Range | 20-2000 U/mL |
| Precision | inter-assay CV: 6.5% | intra-assay CV: 5.4% |
| Sample Volume | 50 μL(tissue homogenate) |
| Assay Time | 1 h 10 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| 20% Rat ileum tissue homogenate | 1 |
| 20% Rat stomach tissue homogenate | 1 |
| 20% Rat liver tissue homogenate | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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