STOML2 Polyclonal Antibody (E-AB-18689)
For research use only.
| Verified Samples |
Verified Samples in WB: Mouse brain, Rat heart, Rat brain Verified Samples in IHC: Human prostate cancer |
| Dilution | WB 1:500-1:2000, IHC 1:20-1:100 |
| Isotype | IgG |
| Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC |
| Clonality | Polyclonal |
| Immunogen | Fusion protein of human STOML2 |
| Abbre | STOML2 |
| Synonyms | EPB72 like protein 2, EPB72-like protein 2, HSPC108, OTTHUMP00000021320, Paraprotein target 7, Paratarg 7, SLP 2, SLP-2, SLP2, STML2, STOML, Stomatin (EPB72) like 2, Stomatin like 2, Stomatin like protein 2, Stomatin-like protein 2, mitochondrial |
| Swissprot | |
| Calculated MW | 39 kDa |
| Observed MW |
Refer to figures
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Membrane. Cytoplasm. Cytoplasm>cytoskeleton. Associated with the cytoskeleton. |
| Concentration | 0.84 mg/mL |
| Buffer | Phosphate buffered solution, pH 7.4, containing 0.05% stabilizer and 50% glycerol. |
| Purification Method | Antigen affinity purification |
| Research Areas | Cancer |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended. |
| background | Human stomatin (band 7.2b) is a 31-kDa erythrocyte membrane protein of unknown function but implicated in the control of ion channel permeability,mechanoreception,and lipid domain organization. Stomatin (EPB72)-like 2 (STOML2,synonyms: SLP-2,HSPC108) is a 38.5-kDa protein that is overall approximately 20% similar to human stomatin. STOML2 is also present in mature human erythrocytes ,but lacks a characteristic NH(2)-terminal hydrophobic domain found in other stomatin homologues. STOML2 may link stomatin or other integral membrane proteins to the peripheral cytoskeleton and thereby play a role in regulating ion channel conductances or the organization of sphingolipid and cholesterol-rich lipid rafts. |
Other Clones
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Other Formats
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Unconjugated
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