Starch Phosphorylase (SP) Activity Colorimetric Assay Kit (E-BC-K1205-M)
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For research use only.
| Detection Principle | Starch Phosphorylase (SP) breaks down the substrate to produce α-D-glucose-1 phosphate, which is converted to α-D-glucose-6 phosphate by the enzyme. It also reduces NAD+/NADP+ with the catalysis of dehydrogenase to produce NADH/NADPH, and the absorbance value at 340 nm increases. |
| Sample Type | Plant tissue |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (340 nm) |
| Research Area | Plant Physiology Series |
| Storage | This product can be stored at 2-8°C/-20°C for 12 months with shading light. Please refer to the manual for the specific storage condition of the components. |
| Valid Period | 12 months |
| Sensitivity | 24 U/g |
| Detection Range | 24-1400 U/g |
| Precision | inter-assay CV: 4.2-5.8% | intra-assay CV: 3.7-5.3% |
| Sample Volume | 20 μL(tissue homogenate) |
| Assay Time | 40 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| 10% Potato tissue homogenate | 1 |
| 10% Corn tissue homogenate | 1 |
| 10% Pumpkin seed tissue homogenate | 1 |
| 10% Garlic tissue homogenate | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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