Sphingomyelin (SM) Fluorometric Assay Kit (E-BC-F079)
For research use only.
| Detection Principle | Sphingomyelin (SM) is widely present in cell membranes and plasma lipoproteins. Metabolism of SM is regulated by sphingomyelin synthase and sphingomyelase, whose metabolites include ceramide, sphingosinol and choline phosphate, which are important second messengers. Studies have shown that SM and its metabolites play an important role in liver, lung, and cardiovascular diseases. The principle of this kit is that SM is catalyzed by sphingomyelin enzyme, and its products generate fluorescent substances with the probe under the action of other enzymes. By substituting the fluorescence value into the standard curve, SM content in the sample can be calculated. |
| Sample Type | Serum,Plasma,Animal tissue,Cell |
| Detection Method | Fluorometric method |
| Detection Instrument | Fluorescence microplate reader (Ex/Em=535 nm/587 nm) |
| Research Area | Glycolysis And Lipid Metabolism |
| Storage | This product can be stored at -20°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 0.35 μmol/L |
| Detection Range | 0.35-40 μmol/L |
| Precision | inter-assay CV: 6.0-10.0% | intra-assay CV: 4.9-5.1% |
| Sample Volume | 40 μL |
| Assay Time | 1 h 30 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| 10% Mouse liver tissue homogenate | 15-20 |
| 10% Mouse brain tissue homogenate | 15-20 |
| Human serum | 80-100 |
| Fetal bovine serum | 80-100 |
| Mouse plasma | 80-100 |
| Rat plasma | 80-100 |
| 1×10^6 Jurkat cells | 1 |
| 1×10^6 293T cells | 1 |
| 1×10^6 HL-60 cells | 1 |
| 1×10^6 Hela cells | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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