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For research use only.
Detection Principle | The formation of nitrophenol from the substrate nitropyranoside is catalyzed by sodium-activated β-galactosidase. The increase rate of absorbance value of nitrophenol at 405 nm per unit time was proportional to the sodium concentration. |
Sample Type | Serum,Plasma,Animal tissue |
Detection Method | Colorimetric method |
Detection Instrument | Spectrophotometer (405 nm) |
Research Area | Inorganic Ions, Oxidative Stress |
Storage | This product can be stored at 2-8°C for 12 months with shading light. |
Valid Period | 12 months |
Sensitivity | 0.02 mmol/L |
Detection Range | 0.02-10 mmol/L |
Precision | inter-assay CV: 7.5-8.9% | intra-assay CV: 2.3-4.5% |
Sample Volume | 50 μL |
The recommended dilution factor for different samples is as follows (for reference only):
Sample Type | Dilution Factor |
---|---|
Human serum | 15-25 |
Mouse serum | 15-25 |
Human plasma | 15-25 |
Rat plasma | 15-25 |
10% Mouse liver tissue homogenate | 15-25 |
10% Rat heart tissue homogenate | 15-25 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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