SMAD4 Polyclonal Antibody (E-AB-34159)
For research use only.
| Verified Samples |
Verified Samples in IF: Mouse testis, Rat lung |
| Dilution | IHC 1:100-1:300, IF 1:200-1:1000 |
| Isotype | IgG |
| Host | Rabbit |
| Reactivity | Human, Mouse, Rat, Monkey |
| Applications | IHC-p, IF |
| Clonality | Polyclonal |
| Immunogen | Synthesized peptide derived from the N-terminal region of human Smad4 |
| Abbre | SMAD4 |
| Synonyms | (Small) Mothers Against Decapentaplegic, DPC 4, DPC4, Deleted in Pancreatic Carcinoma, Deleted in Pancreatic Carcinoma 4, Deleted in pancreatic carcinoma locus 4, Deletion target in pancreatic carcinoma 4, JIP, MAD homolog 4, MAD mothers against decapentapl, hSMAD4 |
| Swissprot | |
| Calculated MW | 60 kDa |
| Observed MW |
60 kDa
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Cytoplasm. Nucleus. Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with R-SMAD. |
| Concentration | 1 mg/mL |
| Buffer | Phosphate buffered solution, pH 7.4, containing 0.05% stabilizer, 0.5% protein protectant and 50% glycerol. |
| Purification Method | Affinity purification |
| Research Areas | Cancer, Cardiovascular, Epigenetics and Nuclear Signaling, Metabolism, Signal Transduction, Stem Cells |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended. |
| background | This gene encodes a member of the Smad family of signal transduction proteins. Smad proteins are phosphorylated and activated by transmembrane serine-threonine receptor kinases in response to TGF-beta signaling. The product of this gene forms homomeric complexes and heteromeric complexes with other activated Smad proteins, which then accumulate in the nucleus and regulate the transcription of target genes. This protein binds to DNA and recognizes an 8-bp palindromic sequence (GTCTAGAC) called the Smad-binding element (SBE). The Smad proteins are subject to complex regulation by post-translational modifications. Mutations or deletions in this gene have been shown to result in pancreatic cancer, juvenile polyposis syndrome, and hereditary hemorrhagic telangiectasia syndrome. SMAD4 (SMAD Family Member 4) is a Protein Coding gene. Diseases associated with SMAD4 include Myhre Syndrome and Polyposis, Juvenile Intestinal. Among its related pathways are PEDF Induced Signaling and Validated targets of C-MYC transcriptional repression. GO annotations related to this gene include transcription factor activity, sequence-specific DNA binding and sequence-specific DNA binding. An important paralog of this gene is SMAD9. |
Other Clones
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Unconjugated
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