SHH Polyclonal Antibody (E-AB-93248)
For research use only.
| Verified Samples |
Verified Samples in WB: various cell lines |
| Dilution | WB 1:500-1:2000 |
| Isotype | IgG |
| Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Applications | WB |
| Clonality | Polyclonal |
| Immunogen | Recombinant fusion protein of mouse SHH |
| Abbre | SHH |
| Synonyms | Dsh, Hhg1, Hx, Hxl3, M100081, 9530036O11Rik |
| Swissprot | |
| Observed MW |
45 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Axon, dendrite, endoplasmic reticulum, extracellular region, extracellular space, Golgi apparatus, nucleus, plasma membrane, synaptic vesicle. |
| Concentration | 1 mg/mL |
| Buffer | Phosphate buffered solution, pH 7.4, containing 0.05% stabilizer and 50% glycerol. |
| Purification Method | Affinity purification |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended. |
| background | Mouse Sonic Hedgehog Homolog (SHH) belongs to a three-protein family called Hedgehog. The other twofamily members are Indian Hedgehog (IHH) and Desert Hedgehog (DHH). Hedgehog proteins are key signalingmolecules in embryonic development. SHH is expressed in various embryonic tissues and plays critical roles inregulating the patterning of many systems, such as limbs and brain. SHH also plays an important role in adult,including the division of adult stem cells and the development of certain cancers and other diseases.Mouse Shhis synthesized as a 437 aa precursor that contains a 24 aa signal sequence and a 413 aa mature region. Themature region is autocatalytically processed into a nonglycosylated, 20 kDa, 174 aa N-terminal fragment (Shh-N), and a catalytic-processing,glycosylated, 34 kDa, 239 aa C-terminal fragment. The 20 kDa Shh-N fragment isthe core of the active hedgehog molecule. Mouse Shh-N is 99%, 98%, and 100% aa identical to human, rat andgerbil Shh-N, respectively. |
Other Clones
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Other Formats
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Unconjugated
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