SEC11A Polyclonal Antibody (E-AB-52703)
For research use only.
| Verified Samples |
Verified Samples in WB: TM4, HepG2, A549, Mouse liver Verified Samples in IHC: Human gastric cancer |
| Dilution | WB 1:500-1:2000, IHC 1:40-1:200 |
| Isotype | IgG |
| Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC |
| Clonality | Polyclonal |
| Immunogen | Fusion protein of human SEC11A |
| Abbre | SEC11A |
| Synonyms | 1810012E07Rik, Endopeptidase SP18, Microsomal signal peptidase 18 kDa subunit, SC11A, SEC11 homolog A, SEC11 homolog A (S. cerevisiae), SEC11-like protein 1, SEC11A, SEC11L1, Signal peptidase complex (18kD), Signal peptidase complex catalytic subunit SEC11A, sid2895 |
| Swissprot | |
| Calculated MW | 21 kDa |
| Observed MW |
Refer to figures
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Microsome membrane. Endoplasmic reticulum membrane. |
| Concentration | 0.78 mg/mL |
| Buffer | Phosphate buffered solution, pH 7.4, containing 0.05% stabilizer and 50% glycerol. |
| Purification Method | Antigen affinity purification |
| Research Areas | Cell Biology |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended. |
| background | This gene encodes a member of the peptidase S26B family. The encoded protein is an 18kDa subunit of the signal peptidase complex and has been linked to cell migration and invasion, gastric cancer and lymph node metastasis. Alternative splicing results in multiple transcript variants. A related pseudogene has been identified on chromosome 8. |
Other Clones
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Unconjugated
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