SCRN2 Polyclonal Antibody (E-AB-52745)
For research use only.
| Verified Samples |
Verified Samples in WB: Mouse small intestines Verified Samples in IHC: Human esophagus cancer, Human tonsil |
| Dilution | WB 1:500-1:2000, IHC 1:50-1:300 |
| Isotype | IgG |
| Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC |
| Clonality | Polyclonal |
| Immunogen | Fusion protein of human SCRN2 |
| Abbre | SCRN2 |
| Synonyms | SCRN2, Secernin 2, Secernin-2, Ses2, scrn2 |
| Swissprot | |
| Calculated MW | 47 kDa |
| Observed MW |
Refer to figures
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Extracellular region or secreted, extracellular exosome. |
| Concentration | 0.84 mg/mL |
| Buffer | Phosphate buffered solution, pH 7.4, containing 0.05% stabilizer and 50% glycerol. |
| Purification Method | Antigen affinity purification |
| Research Areas | Cell Biology |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended. |
| background | The SCRN (Secernin) gene family has three vertebrate paralogs, i.e. SCRN1, SCRN2 and SCRN3, which are closely linked to human HOXA, HOXB and HOXD cluster, respectively. SCRN2 (secernin-2) is a 425 amino acid protein that belongs to the peptidase C69 family and the Secernin subfamily. Vertebrate SCRN genes showed a topology of the form (A)(BC), i.e. (Hsa2 Hsa7)(Hsa17), with SCRN2 falling outside the SCRN3–SCRN1 cluster. The SCRN2 gene is conserved in dog, cow, mouse, rat and zebrafish, and maps to human chromosome 17q21.32. Chromosome 17 makes up over 2.5% of the human genome with about 81 million bases encoding over 1,200 genes. Chromosome 17 is linked to neurofibromatosis, a condition characterized by neural and epidermal lesions, and dysregulated Schwann cell growth. Alexander disease, Birt-Hogg-Dube syndrome and Canavan disease are also associated with chromosome 17. |
Other Clones
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Other Formats
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Unconjugated
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