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For research use only.

Verified Samples Verified Samples in WB: Human stomach
Verified Samples in IHC: Human cervical cancer
Dilution WB 1:500-1:2000,  IHC 1:25-1:100
Isotype IgG
Host Rabbit
Reactivity Human
Applications WB,  IHC
Clonality Polyclonal
Immunogen Full length fusion protein
Abbre SAA4
Synonyms 4,   serum,  Amyloid A,  C SAA,  C-SAA,  CSAA,  Constitutively expressed serum amyloid A protein,  SAA 4,  SAA4 protein,  SAA4_HUMAN,  Saa4,  Serum amyloid A 4 protein,  Serum amyloid A-4 protein,  Serum amyloid A4 constitutive
Swissprot
Calculated MW 15 kDa
Observed MW Refer to figures
The actual band is not consistent with the expectation.

Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include:

1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein.

2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes.

3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1.

4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids).

5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers.

If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane.

Cellular Localization HDL, Secreted
Concentration 0.6 mg/mL
Buffer Phosphate buffered solution, pH 7.4, containing 0.05% stabilizer and 50% glycerol.
Purification Method Antigen affinity purification
Research Areas Cardiovascular,  Metabolism
Conjugation Unconjugated
Storage Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Shipping The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended.
background SAA4 (Serum Amyloid A4, Constitutive) is a Protein Coding gene. Diseases associated with SAA4 include Unicentric Castleman Disease and Amyloidosis Aa. Among its related pathways are Folate Metabolism. Gene Ontology (GO) annotations related to this gene include chemoattractant activity. An important paralog of this gene is SAA2-SAA4.
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Unconjugated

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