S100A1 Polyclonal Antibody (AN006750L)
For research use only.
| Verified Samples | Verified Samples in WB: Mouse heart |
| Dilution | WB 1:250-1:500 |
| Isotype | IgG |
| Host | Rabbit |
| Reactivity | Mouse |
| Applications | WB |
| Clonality | Polyclonal |
| Immunogen | Recombinant Human S100A1 protein expressed by E.coli |
| Abbre | S100A1 |
| Synonyms | S100A, S100A1, Bpb, NEF, Protein S100-A1, S100, S100 alpha, S100 beta, S100 calcium binding protein A1, S100 calcium binding protein B, S100 calcium-binding protein A1, S-100 protein alpha chain, S100 protein alpha polypeptide, S-100 protein subunit alpha, S100-alpha, S100B, S100beta, S100A1 |
| Swissprot | |
| Calculated MW | 11 kDa |
| Observed MW |
12 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Cytoplasm, Sarcoplasmic reticulum, Mitochondrion |
| Tissue Specificity | Highly prevalent in heart. Also found in lesser quantities in skeletal muscle and brain. |
| Concentration | 1 mg/mL |
| Buffer | PBS with 0.05% proclin 300, 1% protective protein and 50% glycerol,pH7.4 |
| Purification Method | Antigen Affinity Purification |
| Research Areas | Neuroscience, Signal Transduction, Tags & Cell Markers |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | The product is shipped with ice pack, upon receipt, store it immediately at the temperature recommended. |
| background | Small calcium binding protein that plays important roles in several biological processes such as Ca2+ homeostasis, chondrocyte biology and cardiomyocyte regulation. In response to an increase in intracellular Ca2+ levels, binds calcium which triggers conformational changes .These changes allow interactions with specific target proteins and modulate their activity.Regulates a network in cardiomyocytes controlling sarcoplasmic reticulum Ca2+ cycling and mitochondrial function through interaction with the ryanodine receptors RYR1 and RYR2, sarcoplasmic reticulum Ca2+-ATPase/ATP2A2 and mitochondrial F1-ATPase. Facilitates diastolic Ca2+ dissociation and myofilament mechanics in order to improve relaxation during diastole. |
Other Clones
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Unconjugated
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