RRP8 Polyclonal Antibody (E-AB-19047)
For research use only.
| Verified Samples |
Verified Samples in WB: Human cerebella, K562 Verified Samples in IHC: Human esophagus cancer, Human breast cancer |
| Dilution | WB 1:500-1:2000, IHC 1:50-1:300 |
| Isotype | IgG |
| Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC |
| Clonality | Polyclonal |
| Immunogen | Fusion protein of human RRP8 |
| Abbre | RRP8 |
| Synonyms | Cerebral protein 1, KIAA0409, NML, Nucleomethylin, Ribosomal RNA processing 8, Ribosomal RNA processing 8 methyltransferase homolog, Ribosomal RNA processing protein 8, Ribosomal RNA-processing prot, homolog (yeast), hypothetical protein LOC23378, methyltransferase |
| Swissprot | |
| Calculated MW | 51 kDa |
| Observed MW |
Refer to figures
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Nucleus>nucleolus. Localizes at rDNA locus. |
| Concentration | 1.44 mg/mL |
| Buffer | Phosphate buffered solution, pH 7.4, containing 0.05% stabilizer and 50% glycerol. |
| Purification Method | Antigen affinity purification |
| Research Areas | Cell Biology |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended. |
| background | RRP8 (Ribosomal RNA Processing 8, Methyltransferase, Homolog (Yeast)) is a Protein Coding gene. Diseases associated with RRP8 include Breast Abscess and Bowen-Conradi Syndrome. Among its related pathways are Activated PKN1 stimulates transcription of AR (androgen receptor) regulated genes KLK2 and KLK3 and Gene Expression. GO annotations related to this gene include poly(A) RNA binding and methylated histone binding. |
Other Clones
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Unconjugated
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