RRP1 Polyclonal Antibody (E-AB-52643)
For research use only.
| Verified Samples |
Verified Samples in WB: 293T, Jurkat Verified Samples in IHC: Human lung cancer |
| Dilution | WB 1:500-1:2000, IHC 1:40-1:200 |
| Isotype | IgG |
| Host | Rabbit |
| Reactivity | Human |
| Applications | WB, IHC |
| Clonality | Polyclonal |
| Immunogen | Fusion protein of human RRP1 |
| Abbre | RRP1 |
| Synonyms | 52-KD, D21S2056E , NNP 1 , NNP-1, NNP1 , NOP52 , Nnp1 homolog nucleolar protein, Novel nuclear protein 1, Nucleolar protein, Nucleolar protein Nop52, Protein NNP 1 , Protein NNP1 , Ribosomal RNA processi, Ribosomal RNA processing 1, Ribosomal RNA processing 1 homolog |
| Swissprot | |
| Calculated MW | 53 kDa |
| Observed MW |
Refer to figures
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Nucleus>nucleolus. |
| Concentration | 0.72 mg/mL |
| Buffer | Phosphate buffered solution, pH 7.4, containing 0.05% stabilizer and 50% glycerol. |
| Purification Method | Antigen affinity purification |
| Research Areas | Cell Biology, Epigenetics and Nuclear Signaling |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended. |
| background | The protein encoded by this gene is the putative homolog of the yeast ribosomal RNA processing protein RRP1. The encoded protein is involved in the late stages of nucleologenesis at the end of mitosis, and may be required for the generation of 28S rRNA. |
Other Clones
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Unconjugated
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