RHAG Polyclonal Antibody (E-AB-19025)
For research use only.
| Verified Samples |
Verified Samples in WB: 293T, K562, HepG2 Verified Samples in IHC: Human tonsil, Human cervical cancer |
| Dilution | WB 1:1000-1:5000, IHC 1:50-1:300 |
| Isotype | IgG |
| Host | Rabbit |
| Reactivity | Human, Mouse |
| Applications | WB, IHC |
| Clonality | Polyclonal |
| Immunogen | Fusion protein of human RHAG |
| Abbre | RHAG |
| Synonyms | Abnormal Rhesus blood group associated glycoprotein, Ammonium transporter Rh type A, CD241, CD241 antigen, Erythrocyte membrane glycoprotein Rh50, Erythrocyte plasma membrane 50 kDa glycoprotein, Rh associated glycoprotein, Rh family type A glycoprotein, Rh type |
| Swissprot | |
| Calculated MW | 44 kDa |
| Observed MW |
Refer to figures
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Cell membrane, multi-pass membrane protein. |
| Concentration | 2.34 mg/mL |
| Buffer | Phosphate buffered solution, pH 7.4, containing 0.05% stabilizer and 50% glycerol. |
| Purification Method | Antigen affinity purification |
| Research Areas | Cardiovascular |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended. |
| background | The protein encoded by this gene is erythrocyte-specific and is thought to be part of a membrane channel that transports ammonium and carbon dioxide across the blood cell membrane. The encoded protein appears to interact with Rh blood group antigens and Rh30 polypeptides. Defects in this gene are a cause of regulator type Rh-null hemolytic anemia (RHN), or Rh-deficiency syndrome.RHAG (Rh-Associated Glycoprotein) is a Protein Coding gene. Diseases associated with RHAG include Anemia, Hemolytic, Rh-Null, Regulator Type and Stomatocytosis I. Among its related pathways are Transport of glucose and other sugars, bile salts and organic acids, metal ions and amine compounds and Erythrocytes take up carbon dioxide and release oxygen. GO annotations related to this gene include ankyrin binding and ammonium transmembrane transporter activity. An important paralog of this gene is RHCG. |
Other Clones
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Other Formats
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Unconjugated
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