Recombinant ZNF364 Monoclonal Antibody (AN301197L)
For research use only.
| Verified Samples | Verified Samples in WB: RAW 264.7 |
| Dilution | WB 1:1000-1:5000, |
| Isotype | IgG,κ |
| Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Applications | WB |
| Clonality | Monoclonal;Recombinant |
| Immunogen | Recombinant Human ZNF364 protein |
| Abbre | ZNF364 |
| Synonyms | BCA, RNF, ZNF, RING finger protein, Zinc finger protein, Breast cancer associated gene, E3 ubiquitin-protein ligase RNF, Rabring, BCA2, ZNF364, RNF115, Breast cancer associated gene 2, CL469780, E3 ubiquitin-protein ligase RNF115, Rabring 7, RING finger protein 115, RN115, Zinc finger protein 364 |
| Swissprot | |
| Calculated MW | 33 kDa |
| Observed MW |
33 kDa
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Cytoplasm {ECO:0000269|PubMed:20019814, ECO:0000269|PubMed:24852021}. Nucleus {ECO:0000269|PubMed:24852021}. Endoplasmic reticulum {ECO:0000269|PubMed:35343654}. Golgi apparatus {ECO:0000269|PubMed:35343654}. Note: The GTP-bound form of RAB7A recruits RNF115 from the cytosol onto late endosomes/lysosomes. {ECO:0000269|PubMed:20019814, ECO:0000269|PubMed:24852021}. |
| Concentration | 0.2 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A |
| Research Areas | Tags & Cell Markers, Cell Biology, Cancer |
| Clone No. | 5D10 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | Enables ubiquitin protein ligase activity. Involved in negative regulation of signal transduction; protein ubiquitination; and ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway. Is active in endoplasmic reticulum. |
Other Clones
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Unconjugated
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