Recombinant ZIKV (strain Zika SPH2015) NS1 protein (C-His Tag) (PKSV030273)
For research use only.
| Synonyms | NS1 protein, Zika virus, strain Zika SPH2015 |
| Species | ZIKV |
| Expression Host | Baculovirus-Insect Cells |
| Sequence | Val796-Leu1157 |
| Accession | ALU33341.1 |
| Calculated Molecular Weight | 42.6 kDa |
| Tag | C-His |
| Bio-activity | Not validated for activity |
| Purity | > 90 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation |
Lyophilized from sterile 20 mM Tris, 500 mM NaCl, pH 8.0, 10% glycerol. Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | Please refer to the printed manual for detailed information. |
| Background | Zika virus NS1 antigen is one of seven non-structural proteins. NS1 is involved in RNA replicaiton. The possible effects of NS1 on hosts include: localization to host cell surface and secreted extracellularly, modelates signalling of innate immune system, has possible damages to platelets and endothelial cells through anti-NS1 antibodies. |
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