Recombinant VEGFA Monoclonal Antibody (AN300844L)
For research use only.
| Verified Samples |
Verified Samples in WB: SH-SY5Y Verified Samples in IHC: Rat heart |
| Dilution | IHC 1:1000-1:5000, WB 1:2000-1:10000 |
| Isotype | IgG,κ |
| Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC |
| Clonality | Monoclonal;Recombinant |
| Immunogen | Recombinant Human VEGFA protein |
| Abbre | VEGFA |
| Synonyms | MVCD, Vascular Endothelial Growth Factor Isoform, VEGFA, MVCD1, VEGF, VPF, L VEGFA, VEGF A, Folliculostellate cell-derived growth factor, Glioma-derived endothelial cell mitogen, Vascular Endothelial Growth Factor Isoform 165, Vascular permeability factor, VEGF121, VEGF165, VEGF-165, VEGF-A, Vascular endothelial growth factor A, Vascular permeability factor (VPF), MVCD1, vascular endothelial growth factor A, VEGF, VPF, MGC70609, vascular endothelial growth factor, vascular endothelial growth factor A121, vascular endothelial growth factor A165, VEGF120, VEGF-A |
| Swissprot | |
| Calculated MW | 27 kDa |
| Observed MW |
40 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Secreted |
| Concentration | 0.2 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A |
| Research Areas | Cardiovascular, Signal Transduction, Cancer, Developmental Biology, Kits, Lysates, Other, Metabolism |
| Clone No. | 5H3 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | This gene is a member of the PDGF/VEGF growth factor family. It encodes a heparin-binding protein, which exists as a disulfide-linked homodimer. This growth factor induces proliferation and migration of vascular endothelial cells, and is essential for both physiological and pathological angiogenesis. Disruption of this gene in mice resulted in abnormal embryonic blood vessel formation. This gene is upregulated in many known tumors and its expression is correlated with tumor stage and progression. Elevated levels of this protein are found in patients with POEMS syndrome, also known as Crow-Fukase syndrome. Allelic variants of this gene have been associated with microvascular complications of diabetes 1 (MVCD1) and atherosclerosis. Alternatively spliced transcript variants encoding different isoforms have been described. |
Other Clones
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Unconjugated
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