Recombinant UCHL1/PGP9.5 Monoclonal Antibody (AN300102P)
For research use only.
| Verified Samples |
Verified Samples in WB: SH-SY5Y Verified Samples in IF: DU145 |
| Dilution | WB 1:500-1:2000, ICC/IF 1:20-1:100, IP 0.5-2 μL/mg of lysate |
| Isotype | IgG |
| Host | Rabbit |
| Reactivity | Human |
| Applications | WB, ICC/IF, IP |
| Clonality | Rabbit Monoclonal |
| Immunogen | Recombinant Human UCHL1 protein |
| Abbre | UCHL1 |
| Synonyms | SPG, HEL, PARK, UCHL, Ubiquitin thioesterase L, Neuron Cytoplasmic Protein, Ubiquitin carboxyl-terminal hydrolase isozyme L, HEL-S, Uch-L, PGP, HEL-117, HEL-S-53, NDGOA, PARK5, PGP 9.5, PGP9.5, PGP95, SPG79, Uch-L1, UCHL1, Neuron Cytoplasmic Protein 9.5, Ubiquitin carboxyl-terminal hydrolase isozyme L1, Ubiquitin thioesterase L1, UCHL-1, EC:3.4.19.12, Epididymis luminal protein 117, Epididymis secretory protein Li 53, HEL 117, HEL S 53, OTTHUMP00000218137, OTTHUMP00000218139, OTTHUMP00000218140, OTTHUMP00000218141, Park 5, PGP 9.5 (PGP9.5), Protein gene product 9.5, Ubiquitin C terminal esterase L1, Ubiquitin C terminal hydrolase, Ubiquitin C terminal hydrolase L1, Ubiquitin carboxyl terminal esterase L1, Ubiquitin carboxyl terminal hydrolase isozyme L1, Ubiquitin C-terminal hydrolase L1, Ubiquitin thiolesterase, Ubiquitin thiolesterase L1, NDGOA, Neuron cytoplasmic protein 9.5, PARK5, PGP 9.5, PGP9.5, PGP95, Ubiquitin carboxyl-terminal hydrolase isozyme L1, Ubiquitin thioesterase L1, UCH-L1, UCHL1 |
| Swissprot | |
| Calculated MW | 25 kDa |
| Observed MW |
24 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Cytoplasm, Endoplasmic reticulum membrane |
| Tissue Specificity | Neuronal cell, Neocortex. |
| Concentration | 1 mg/mL |
| Buffer | 0.2 μm filtered solution in PBS |
| Purification Method | Protein A |
| Research Areas | Neuroscience, Cell Biology, Tags & Cell Markers |
| Clone No. | 11G6 |
| Conjugation | Unconjugated |
| Storage | This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles. |
| Shipping | Ice bag |
| background | Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins (Probable). This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer may have ATP-independent ubiquitin ligase activity. |
Other Clones
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Unconjugated
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