Recombinant Transmembrane Protein 173 Monoclonal Antibody (E-AB-81615)
For research use only.
| Verified Samples |
Verified Samples in WB: K562, Rat Rat Brain Verified Samples in IHC: Human tonsil |
| Dilution | WB 1:500-1:1000, IHC 1:50-1:100 |
| Isotype | IgG |
| Host | Rabbit |
| Reactivity | Human, Rat |
| Applications | WB, IHC-P |
| Clonality | Rabbit Monoclonal |
| Immunogen | Recombinant protein of human TMEM173 |
| Abbre | Transmembrane Protein 173 |
| Synonyms | ERIS, Endoplasmic reticulum interferon stimulator, FLJ38577, MITA, MPYS, Mediator of IRF3 activation, Mitochondrial mediator of IRF3 activation, N terminal methionine proline tyrosine serine plasma membrane tetra, endoplasmic reticulum IFN stimulator, hMITA, hSTING |
| Swissprot | |
| Calculated MW | 42 kDa |
| Observed MW |
40 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Endoplasmic reticulum membrane. Mitochondrion outer membrane. Cell membrane. Cytoplasm>perinuclear region. In response to double-stranded DNA stimulation, relocalizes to perinuclear region, where the kinase TBK1 is recruited. |
| Concentration | 300 μg/mL |
| Buffer | 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% stabilizer and 0.05% protective protein. |
| Purification Method | Affinity Purified |
| Research Areas | Cancer, Epigenetics and Nuclear Signaling, Immunology, Signal Transduction |
| Clone No. | R06-9A7 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended. |
| background | This gene encodes a five transmembrane protein that functions as a major regulator of the innate immune response to viral and bacterial infections. The encoded protein is a pattern recognition receptor that detects cytosolic nucleic acids and transmits signals that activate type I interferon responses. The encoded protein has also been shown to play a role in apoptotic signaling by associating with type II major histocompatibility complex. Mutations in this gene are the cause of infantile-onset STING-associated vasculopathy. Alternate splicing results in multiple transcript variants. |
Other Clones
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Unconjugated
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