Recombinant Thrombospondin 1 Monoclonal Antibody (E-AB-81612)
For research use only.
| Verified Samples |
Verified Samples in WB: MCF-7, 293T, C2C12 Verified Samples in IHC: Human Cholangiocarcinoma |
| Dilution | WB 1:500-1:1000, IHC 1:50-1:100 |
| Isotype | IgG |
| Host | Rabbit |
| Reactivity | Human, Mouse |
| Applications | WB, IHC-P |
| Clonality | Rabbit Monoclonal |
| Immunogen | Recombinant protein of human Thrombospondin 1 |
| Abbre | Thrombospondin 1 |
| Synonyms | THBS, THBS 1, TSP, TSP 1, TSP1, Thbs1, Thrombospondin 1, Thrombospondin-1 |
| Swissprot | |
| Calculated MW | 130 kDa |
| Observed MW |
170 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Endoplasmic reticulum, endoplasmic reticulum, endoplasmic reticulum lumen, sarcoplasmic reticulum, Extracellular region or secreted, extracellular exosome, extracellular matrix, extracellular region, extracellular space, fibrinogen complex, Plasma Membrane, external side of plasma membrane, Other locations: cell surface, platelet alpha granule, platelet alpha granule lumen, secretory granule. |
| Concentration | 300 μg/mL |
| Buffer | 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% stabilizer and 0.05% protective protein. |
| Purification Method | Affinity Purified |
| Research Areas | Cancer, Cardiovascular, Metabolism, Neuroscience, Stem Cells |
| Clone No. | R06-5F3 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended. |
| background | The protein encoded by this gene is a subunit of a disulfide-linked homotrimeric protein. This protein is an adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. This protein can bind to fibrinogen, fibronectin, laminin, type V collagen and integrins alpha-V/beta-1. This protein has been shown to play roles in platelet aggregation, angiogenesis, and tumorigenesis. |
Other Clones
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Other Formats
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Unconjugated
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