Recombinant TGF β1 Monoclonal Antibody (AN300941L)
For research use only.
| Verified Samples |
Verified Samples in WB: HepG2 Verified Samples in IHC: Rat jejunum |
| Dilution | IHC 1:2000-1:5000, WB 1:1000-1:5000 |
| Isotype | IgG,κ |
| Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC |
| Clonality | Monoclonal;Recombinant |
| Immunogen | Recombinant Human TGF β1 protein |
| Abbre | TGF β1 |
| Synonyms | DPD, Transforming growth factor beta, TGF-B, TGFB1, CED, DPD1, LAP, TGFB, TGFbeta, TGF-β, Cartilage-inducing factor, Differentiation inhibiting factor, IBDIMDE, TGF-B1, TGF-beta 1, TGF-beta-1, TGF-β1, Transforming growth factor beta-1 proprotein, Latency-associated peptide, Transforming growth factor beta-1, beta 1, Prepro transforming growth factor beta 1, TGF beta, TGF beta 1, TGF beta 1 protein, Tgfb-1, TGF-beta 1 protein, TGFbeta1, TGF-beta1, TGF-beta-5, transforming growth factor, Transforming Growth Factor b1, Transforming Growth Factor beta 1, Transforming growth factor beta 1a, Transforming Growth Factor-1, LAP |
| Swissprot | |
| Calculated MW | 44 kDa |
| Observed MW |
44 kDa,13 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Secreted |
| Tissue Specificity | Bone, articular cartilage and chondrocytes. |
| Concentration | 0.2 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A |
| Research Areas | Signal Transduction, Stem Cells, Cancer, Metabolism, Cardiovascular, Cell Biology |
| Clone No. | 3F3 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | Transforming growth factor beta 1(TGFB1) Homo sapiens This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate a latency-associated peptide (LAP) and a mature peptide, and is found in either a latent form composed of a mature peptide homodimer, a LAP homodimer, and a latent TGF-beta binding protein, or in an active form consisting solely of the mature peptide homodimer. The mature peptide may also form heterodimers with other TGFB family members. This encoded protein regulates cell proliferation, differentiation and growth, and can modulate expression and activation of other growth factors including interferon gamma and tumor necrosis factor alpha. |
Other Clones
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