Recombinant TATA Box Binding Protein Monoclonal Antibody (AN301051L)
For research use only.
| Verified Samples |
Verified Samples in WB: HEK293 Verified Samples in IHC: Human colon carcinoma tissue, Mouse colon tissue |
| Dilution | IHC 1:400-1000, WB 1:1000-5000 |
| Isotype | IgG,κ |
| Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC |
| Clonality | Monoclonal;Recombinant |
| Immunogen | Recombinant Human TATA Box Binding Protein protein |
| Abbre | TATA Box Binding Protein |
| Synonyms | HDL, SCA, TBP, GTF2D, GTF2D1, HDL4, SCA17, TFIID, TATA sequence-binding protein, Transcription initi, TF2D, GTF2D1, HDL4, SCA17, TATA sequence-binding protein, TBP, TFIID, BA2R, CCG1, CCGS, DYT3, DYT3/KAT4, MRXS33, N-NSCL2, P250, TAF(II)250, TAF1, TAF2A, TAFII250, TAFII-250, TATA binding factor, TATA box binding protein, TATA box factor, TATA sequence binding protein, TATA-binding factor, TATA-binding protein, TATA-box binding protein, TATA-box binding protein associated factor 1, TATA-box factor, TATA-box-binding protein, Transcription initiation factor TFIID TBP subunit, XDP, TATA Binding Protein |
| Swissprot | |
| Calculated MW | 38 kDa |
| Observed MW |
38 kDa
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Nucleus |
| Tissue Specificity | Widely expressed, with levels highest in the testis and ovary. |
| Concentration | 0.2 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A |
| Research Areas | Epigenetics and Nuclear Signaling, Isotype, Loading Controls |
| Clone No. | 7F14 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes TBP, the TATA-binding protein. A distinctive feature of TBP is a long string of glutamines in the N-terminus. |
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