Recombinant Sumo 1 Monoclonal Antibody (AN300863L)
For research use only.
| Verified Samples |
Verified Samples in WB: Jurkat Verified Samples in IHC: Human lung carcinoma tissue |
| Dilution | IHC 1:2000-1:10000, WB 1:2000-1:10000 |
| Isotype | IgG,κ |
| Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC |
| Clonality | Monoclonal;Recombinant |
| Immunogen | Recombinant Human Sumo 1 protein |
| Abbre | Sumo 1 |
| Synonyms | UBL, SMT, OFC, PIC, DAP, GMP, Senp, small ubiquitin-like modifier, SMT3H, OK/SW-cl, DAP1, GMP1, OFC10, PIC1, SENP2, SMT3, SMT3C, SMT3H3, UBL1, SUMO1, SUMO-1, small ubiquitin-like modifier 1, OK/SW-cl.43, DAP1, GMP1, OFC10, PIC1, SENP2, small ubiquitin-like modifier 1, SMT3, SMT3C, SMT3H3, UBL1, GAP modifying protein 1, GAP-modifying protein 1, GMP 1, homolog 3, PIC 1, Sentrin, Sentrin 1, Small ubiquitin related modifier 1, Small ubiquitin-related modifier 1, SMT3 homolog 3, SMT3 suppressor of mif two 3 homolog 1, Ubiquitin homology domain protein PIC1, Ubiquitin Like 1, Ubiquitin like protein SMT3C, Ubiquitin like protein UBL1, Ubiquitin-homology domain protein PIC1, Ubiquitin-Like Protein, Ubiquitin-like protein SMT3C, Ubiquitin-like protein UBL1, UBL 1, yeast, SUMO-1 |
| Swissprot | |
| Calculated MW | 12 kDa |
| Observed MW |
80 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Cytoplasm, Nucleus |
| Concentration | 0.2 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A |
| Research Areas | Cell Biology, Cardiovascular, Metabolism, Cancer |
| Clone No. | 8B1 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | This gene encodes a protein that is a member of the SUMO (small ubiquitin-like modifier) protein family. It functions in a manner similar to ubiquitin in that it is bound to target proteins as part of a post-translational modification system. However, unlike ubiquitin which targets proteins for degradation, this protein is involved in a variety of cellular processes, such as nuclear transport, transcriptional regulation, apoptosis, and protein stability. It is not active until the last four amino acids of the carboxy-terminus have been cleaved off. Several pseudogenes have been reported for this gene. Alternate transcriptional splice variants encoding different isoforms have been characterized. |
Other Clones
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