Recombinant STAT1 α Monoclonal Antibody (AN301097L)
For research use only.
| Verified Samples |
Verified Samples in WB: A549 Verified Samples in IHC: Human kidney tissue |
| Dilution | IHC 1:500-1000, WB 1:1000-5000 |
| Isotype | IgG,κ |
| Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC |
| Clonality | Monoclonal;Recombinant |
| Immunogen | Recombinant Human STAT1 α protein |
| Abbre | STAT1 α |
| Synonyms | ISGF, Transcription Factor ISGF-3 Components p91/p, CANDF, CANDF7, IMD31A, IMD31B, IMD31C, ISGF-3, STAT91, STAT1 alpha, STAT1, Signal Transducer and Activator of Transcription 1-Alpha/Beta, Transcription Factor ISGF-3 Components p91/p84, 91kD, DKFZp686B04100, ISGF 3, OTTHUMP00000163552, OTTHUMP00000165046, OTTHUMP00000165047, OTTHUMP00000205845, Signal transducer and activator of transcription 1, Signal transducer and activator of transcription 1 91kD, Signal transducer and activator of transcription 1 91kDa, STAT 1, Transcription factor ISGF 3 components p91 p84, STAT1 |
| Swissprot | |
| Calculated MW | 87 kDa |
| Observed MW |
90 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Nuclear |
| Concentration | 0.2 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A |
| Research Areas | Signal Transduction, Epigenetics and Nuclear Signaling, Cancer |
| Clone No. | 6A6 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | STAT1(signal transducer and activator of transcription 1) Homo sapiens The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein can be activated by various ligands including interferon-alpha, interferon-gamma, EGF, PDGF and IL6. This protein mediates the expression of a variety of genes, which is thought to be important for cell viability in response to different cell stimuli and pathogens. Two alternatively spliced transcript variants encoding distinct isoforms have been described. |
Other Clones
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Unconjugated
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