For research use only.
| Verified Samples | Verified Samples in WB: 3T3, Hela Verified Samples in IHC: Human breast cancer |
| Dilution | WB 1:1000-1:2000, IHC 1:20-1:100 |
| Isotype | IgG |
| Host | Rabbit |
| Reactivity | Human, Mouse |
| Applications | WB, IHC-P |
| Clonality | Rabbit Monoclonal |
| Immunogen | Recombinant protein of human SOX9 |
| Abbre | SOX9 |
| Synonyms | CMD 1, CMD1, CMPD 1, CMPD1, SOX 9, SOX9, SRA 1, SRA1, SRXX2, SRXY10, SRY , SRY (sex determining region Y) box 9, SRY (sex determining region Y) box 9 (campomelic dysplasia autosomal, SRY (sex determining region Y)-box 9, Sox9, campomelic dysplasia autosomal sex reversal |
| Swissprot | |
| Calculated MW | 56 kDa |
| Observed MW | 70 kDa The actual band is not consistent with the expectation. Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Nucleus. |
| Concentration | 300 μg/mL |
| Buffer | 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% stabilizer and 0.05% protective protein. |
| Purification Method | Affinity Purified |
| Research Areas | Cancer, Epigenetics and Nuclear Signaling, Developmental Biology, Neuroscience, Stem Cells |
| Clone No. | R06-7F6 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended. |
| background | The protein encoded by this gene recognizes the sequence CCTTGAG along with other members of the HMG-box class DNA-binding proteins. It acts during chondrocyte differentiation and, with steroidogenic factor 1, regulates transcription of the anti-Muellerian hormone (AMH) gene. Deficiencies lead to the skeletal malformation syndrome campomelic dysplasia, frequently with sex reversal. |
Other Clones
1 Results
Other Formats
1 Results
Unconjugated
