Recombinant Sodium Potassium ATPase Monoclonal Antibody (AN300610L)
For research use only.
| Verified Samples | Verified Samples in WB: A549 |
| Dilution | WB 1:20000-1:50000 |
| Isotype | IgG,κ |
| Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Applications | WB |
| Clonality | Monoclonal;Recombinant |
| Immunogen | Recombinant Human Sodium Potassium ATPase protein |
| Abbre | Sodium Potassium ATPase |
| Synonyms | ATPase Na+/K+ transporting subunit alpha, HOMGSMR, ATP1A1, ATPase Na+/K+ transporting subunit alpha 1, ATP1A, CMT2DD, HOMGSMR2, A1A1, AT1A1, Atpa-1, ATPase Na+/K+ transporting alpha 1 polypeptide, BC010319, EC 3.6.3.9, K ATPase alpha 1 subunit, K+ ATPase alpha subunit, MGC3285, MGC38419, MGC51750, Na K ATPase alpha A catalytic polypeptide, Na K ATPase catalytic subunit alpha A protein, Na(+)/K(+) ATPase 1, Na(+)/K(+) ATPase alpha-1 subunit, Na+, Na+/K+ ATPase alpha 1 subunit, Na+/K+ ATPase 1, Nkaa1b, Sodium potassium ATPase alpha 1 polypeptide, Sodium pump 1, Sodium pump subunit alpha-1, Sodium/potassium-transporting ATPase subunit alpha-1, sodium-potassium ATPase catalytic subunit alpha-1, Na+/K+-ATPase |
| Swissprot | |
| Calculated MW | 113 kDa |
| Observed MW |
100 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Membranous |
| Concentration | 0.2 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A |
| Research Areas | Tags & Cell Markers, Signal Transduction, Neuroscience, Cancer, Metabolism, Isotype, Loading Controls |
| Clone No. | 3P9 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | ATPase Na+/K+ transporting subunit alpha 1(ATP1A1) Homo sapiens The protein encoded by this gene belongs to the family of P-type cation transport ATPases, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The catalytic subunit of Na+/K+ -ATPase is encoded by multiple genes. This gene encodes an alpha 1 subunit. Multiple transcript variants encoding different isoforms have been found for this gene. |
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Unconjugated
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