Recombinant SIRT3 Monoclonal Antibody (AN300974L)
For research use only.
| Verified Samples |
Verified Samples in WB: U-14 Verified Samples in IHC: Human kidney tissue |
| Dilution | IHC 1:200-1:1000, WB 1:1000-1:5000 |
| Isotype | IgG,κ |
| Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC |
| Clonality | Monoclonal;Recombinant |
| Immunogen | Recombinant Human SIRT3 protein |
| Abbre | SIRT3 |
| Synonyms | SIRT, SIR2L, sirtuin, SIRT3, SIR2L3, sirtuin 3, SIR2L3, sirtuin 3, hSIRT 3, hSIRT3, mitochondrial, Mitochondrial nicotinamide adenine dinucleotide dependent deacetylase, NAD dependent deacetylase sirtuin 3 mitochondrial, NAD-dependent protein deacetylase sirtuin-3, Regulatory protein SIR2 homolog 3, Silent mating type information regulation 2 S.cerevisiae homolog 3, Sir 2 like 3, SIR 2 like protein 3, SIR 3, SIR2 L3, Sir2 like 3, SIR2 like protein 3, SIR2-like protein 3, SIR3, SIRT 3, Sirtuin silent mating type information regulation 2 homolog 3 (S. cerevisiae), Sirtuin type 3, Sirtuin3 |
| Swissprot | |
| Calculated MW | 44 kDa |
| Observed MW |
28 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Mitochondrion matrix |
| Concentration | 0.2 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A |
| Research Areas | Epigenetics and Nuclear Signaling, Cell Biology, Cancer, Cardiovascular, Metabolism |
| Clone No. | 10A9 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class I of the sirtuin family. Two alternatively spliced transcript variants that encode different proteins have been described for this gene. |
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Unconjugated
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