Recombinant SerpinF2 Monoclonal Antibody (AN300314P)
For research use only.
| Verified Samples |
Verified Samples in WB:?HepG2 Verified Samples in IP: HepG2 |
| Dilution | WB 1:500-1:1000, IP 1-2 μL/mg of lysate |
| Isotype | IgG |
| Host | Rabbit |
| Reactivity | Human |
| Applications | WB, IP |
| Clonality | Monoclonal |
| Immunogen | Recombinant Human SerpinF2 Protein |
| Abbre | SerpinF2 |
| Synonyms | PDGFA-Associated Protein, PDAP, HASPP, SERPINF, SERPINF2, A2AP, AAP, ALPHA-2-PI, API, PLI, PAP1, Alpha 2-antiplasmin, Alpha-2-plasmin inhibitor, HASPP28, PAP, PDAP1, PDGFA-Associated Protein 1, plasmin inhibitor, α2-antiplasmin, Alpha 2 antiplasmin, Alpha 2 antiplasmin pigment epithelium derived factor, Alpha 2 AP, ALPHA 2 PI, Alpha 2 plasmin inhibitor, Alpha 2 plasmin inhibitor deficiency, Alpha-2-antiplasmin, Alpha-2-AP, Antiplasmin deficiency, clade F, clade F (alpha 2 antiplasmin pigment epithelium derived factor) member 2, isoform CRA_c, member 2, Plasmin inhibitor deficiency, Serine (or cysteine) peptidase inhibitor, Serine (or cysteine) proteinase inhibitor, Serine or cysteine peptidase inhibitor clade F member 2, Serpin F2, Serpin peptidase inhibitor, Serpin peptidase inhibitor clade F |
| Swissprot | |
| Calculated MW | 56 kDa |
| Observed MW |
60 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Tissue Specificity | Expressed by the liver and secreted in plasma. |
| Concentration | 1 mg/mL |
| Buffer | 0.2 μm filtered solution in PBS |
| Purification Method | Protein A |
| Research Areas | Cardiovascular, Cell Biology |
| Clone No. | 8D1 |
| Conjugation | Unconjugated |
| Storage | This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles. |
| Shipping | Ice bag |
| background | This gene encodes a member of the serpin family of serine protease inhibitors. The protein is a major inhibitor of plasmin, which degrades fibrin and various other proteins. Consequently, the proper function of this gene has a major role in regulating the blood clotting pathway. Mutations in this gene result in alpha-2-plasmin inhibitor deficiency, which is characterized by severe hemorrhagic diathesis. Multiple transcript variants encoding different isoforms have been found for this gene. |
Other Clones
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Unconjugated
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