Recombinant SerpinA8/Angiotensinogen/AGT Monoclonal Antibody (AN300213P)
For research use only.
| Verified Samples |
Verified Samples in WB: HepG2 Verified Samples in FCM: HepG2 Verified Samples in IF: Hela, SKBR3 |
| Dilution | WB 1:500-1:2000, FCM 1:25-1:100, ICC/IF 1:20-1:100, IP 4-6 μL/mg of lysate |
| Isotype | IgG |
| Host | Rabbit |
| Reactivity | Human |
| Applications | WB, FCM, ICC/IF, IP |
| Clonality | Rabbit Monoclonal |
| Immunogen | Recombinant Human SerpinA8 protein |
| Abbre | AGT |
| Synonyms | hFLT, Angiotensin 2, Angiotensin 3, AGT and SERPINA, SERPINA, Serpin A, AGT, ANHU, hFLT1, Aangiotensinogen, AGT and SERPINA8, Alpha 1 antiproteinase antitrypsin, Ang, ANGT, ANRT, AT-2, PAT, Angiotensinogen, Angiotensin-1, SERPINA8, Angiotensin-2, Angiotensin-3, Angiotensin-4, Serpin A8, Angiotensin 1-7, Angiotensin 1-10, Angiotensin I, Ang I, Angiotensin 1-8, Angiotensin II, Ang II, Angiotensin 2-8, Angiotensin III, Ang III, Angiotensin 3-8, Ang IV, Angiotensin 1-9, Angiotensin 1-5, Angiotensin 1-4, Angiotensin IV, Aangiotensinogen (serpin peptidase inhibitor clade A member 8), AI265500, Ang, AngII, Angiotensinogen (PAT), AT-II, Des-Asp[1]-angiotensin II, FLJ92595, FLJ97926, MGC105326, Pre angiotensinogen, Serine (or cysteine) proteinase inhibitor, Serpin peptidase inhibitor clade A member 8 |
| Swissprot | |
| Calculated MW | 51 kDa |
| Observed MW |
51 kDa
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Secreted |
| Concentration | 1 mg/mL |
| Buffer | 0.2 μm filtered solution in PBS |
| Purification Method | Protein A |
| Research Areas | Cardiovascular, Signal Transduction, Metabolism, |
| Clone No. | 9A4 |
| Conjugation | Unconjugated |
| Storage | This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles. |
| Shipping | Ice bag |
| background | Essential component of the renin-angiotensin system (RAS), a potent regulator of blood pressure, body fluid and electrolyte homeostasis. In response to lowered blood pressure, the enzyme renin cleaves angiotensinogen to produce angiotensin-1 (angiotensin 1-10). Angiotensin-1 is a substrate of ACE (angiotensin converting enzyme) that removes a dipeptide to yield the physiologically active peptide angiotensin-2 (angiotensin 1-8). Angiotensin-1 and angiotensin-2 can be further processed to generate angiotensin-3 (angiotensin 2-8), angiotensin-4 (angiotensin 3-8). Angiotensin 1-7 is cleaved from angiotensin-2 by ACE2 or from angiotensin-1 by MME (neprilysin). Angiotensin 1-9 is cleaved from angiotensin-1 by ACE2.Angiotensin-2 acts directly on vascular smooth muscle as a potent vasoconstrictor, affects cardiac contractility and heart rate through its action on the sympathetic nervous system, and alters renal sodium and water absorption through its ability to stimulate the zona glomerulosa cells of the adrenal cortex to synthesize and secrete aldosterone.Angiotensin-3 stimulates aldosterone release.Angiotensin 1-7 is a ligand for the G-protein coupled receptor MAS1 (By similarity). Has vasodilator and antidiuretic effects (By similarity). Has an antithrombotic effect that involves MAS1-mediated release of nitric oxide from platelets. |
Other Clones
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Other Formats
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Unconjugated
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