Recombinant SDHA Monoclonal Antibody (AN301107L)
For research use only.
| Verified Samples |
Verified Samples in WB: Hela Verified Samples in IHC: Human kidney tissue, Rat kidney tissue |
| Dilution | IHC 1:200-1000, WB 1:1000-5000 |
| Isotype | IgG,κ |
| Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC |
| Clonality | Monoclonal;Recombinant |
| Immunogen | Recombinant Human SDHA protein |
| Abbre | SDHA |
| Synonyms | PGL, SDH, SDHA, CMD1GG, FP, PGL5, SDH1, SDH2, SDHF, DHSA_HUMAN, Flavoprotein subunit of complex II, Flavoprotein subunit of complex II (Fp), mitochondrial, SDH 2, Succinate dehydrogenase [ubiquinone] flavoprotein subunit, DHSA, Succinate dehydrogenase [ubiquinone] flavoprotein subunit mitochondrial, Succinate dehydrogenase complex flavoprotein subunit, Succinate dehydrogenase complex flavoprotein subunit A, Succinate dehydrogenase complex flavoprotein subunit precursor, Succinate dehydrogenase complex subunit A, Succinate dehydrogenase complex subunit A flavoprotein, Succinate dehydrogenase complex subunit A flavoprotein (Fp) |
| Swissprot | |
| Calculated MW | 73 kDa |
| Observed MW |
73 kDa
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Cytoplasmic |
| Concentration | 0.2 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A |
| Research Areas | Tags & Cell Markers, Signal Transduction, Cell Biology, Cancer, Metabolism |
| Clone No. | 10F11 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | This gene encodes a major catalytic subunit of succinate-ubiquinone oxidoreductase, a complex of the mitochondrial respiratory chain. The complex is composed of four nuclear-encoded subunits and is localized in the mitochondrial inner membrane. Mutations in this gene have been associated with a form of mitochondrial respiratory chain deficiency known as Leigh Syndrome. A pseudogene has been identified on chromosome 3q29. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. |
Other Clones
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Unconjugated
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