Recombinant SARS-CoV S-trimer Protein (C-6His) (PKSV030289)
For research use only.
| Synonyms | SARS-CoV S glycoprotein, SARS-CoV S-trimer Protein, SARS-CoV Spike glycoprotein, SARS-CoV Sprotein |
| Species | SARS |
| Expression Host | HEK293 Cells |
| Sequence | Ser14-Gln1190 |
| Accession | P59594 |
| Calculated Molecular Weight | 134.9 kDa |
| Observed Molecular Weight | 170-220 kDa |
| Tag | C-His |
| Bio-activity | Immobilized SARS-CoV S-trimer Protein-His(PKSV030289) at 5μg/ml (100 μl/well) can bind Human ACE-2- Fc(PKSR030492). The ED50 of Recombinant Human ACE-2- Fc(PKSR030492) is 33. 26 ng/ml. |
| Purity | > 95 % as determined by reducing SDS-PAGE. |
| Endotoxin | Please contact us for more information. |
| Storage | Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles. |
| Shipping | This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C. |
| Formulation | Supplied as a 0.2 μm filtered solution of PBS, pH7.4. |
| Reconstitution | Not Applicable |
| Background | The spike (S) glycoprotein of coronaviruses contains protrusions that will only bind to certain receptors on the host cell. Known receptors bind S1 are ACE2, angiotensin-converting enzyme 2; DPP4, dipeptidyl peptidase-4; APN, aminopeptidase N; CEACAM, carcinoembryonic antigen-related cell adhesion molecule 1; Sia, sialic acid; O-ac Sia, O-acetylated sialic acid. The spike (S) glycoprotein of coronaviruses is known to be essential in the binding of the virus to the host cell at the advent of the infection process. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain |
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