Recombinant SARS-CoV-2 NSP10 Protein (His Tag) (PKSR030471)
For research use only.
| Synonyms | SARS-CoV 2 GFL, SARS-CoV 2 Growth factor-like peptide, SARS-CoV 2 nsp10 |
| Species | SARS-CoV-2 |
| Expression Host | E.coli |
| Sequence | Ala1-Gln139 |
| Accession | YP_009725306.1 |
| Calculated Molecular Weight | 17.9 kDa |
| Observed Molecular Weight | 18 kDa |
| Tag | N-His |
| Bio-activity | Not validated for activity |
| Purity | > 80 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles. |
| Shipping | This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C. |
| Formulation | Supplied as a 0.2 μM filtered solution of PBS, 10% Glycerol, pH 7.4. |
| Reconstitution | Not Applicable |
| Background | Nsp10 have shown that it is a 15-kDa protein of unknown function that has been shown to interact with itself, nsp1, and nsp7. It colocalizes with N to sites of viral replication and is essential for replication. It plays a pivotal role in viral transcription by stimulating both nsp14 3'-5' exoribonuclease and nsp16 2'-O-methyltransferase activities. Therefore plays an essential role in viral mRNAs cap methylation. Nsp10 is a critical regulator of coronavirus RNA synthesis and may play an important role in polyprotein processing. |
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