Recombinant S100A8 Monoclonal Antibody (AN300036P)
For research use only.
| Verified Samples |
Verified Samples in WB: THP-1 Verified Samples in IP: THP-1 |
| Dilution | WB 1:500-1:2000, IP 5-10 μL/mg of lysate |
| Isotype | IgG |
| Host | Rabbit |
| Reactivity | Human |
| Applications | WB, IP |
| Clonality | Rabbit Monoclonal |
| Immunogen | Recombinant Human S100A8 Protein |
| Abbre | S100A8 |
| Synonyms | Protein S100-A, S100a, Kcnip, KCHIP, MRP, S100A8, 60B8AG, CAGA, CFAG, CGLA, CP-10, L1Ag, MA387, MIF, MRP8, NIF, P8, Calgranulin A, Calgranulin-A, Cystic fibrosis antigen, Leukocyte L1 complex light chain, MRP-8, Protein S100-A8, CALP, KCNIP4, KCHIP4, AI323541, B8Ag, BEE11, Calprotectin, Calprotectin L1L subunit, Chemotactic cytokine CP-10, included, Migration inhibitory factor-related protein 8, Myeloid-related protein 8, Neutrophil cytosolic 7 kDa protein, Pro-inflammatory S100 cytokine, S100 calcium binding protein A8, S100 calcium binding protein A8 (calgranulin A), S100 calcium-binding protein A8, S100A8/S100A9 complex, S10A8, Urinary stone protein band A |
| Swissprot | |
| Calculated MW | 11 kDa |
| Observed MW |
11 kDa
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Concentration | 1 mg/mL |
| Buffer | 0.2 μm filtered solution in PBS |
| Purification Method | Protein A |
| Research Areas | Neuroscience, Cancer, Immunology, Signal Transduction, Cell Biology, Epigenetics and Nuclear Signaling |
| Clone No. | 8D8 |
| Conjugation | Unconjugated |
| Storage | This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles. |
| Shipping | Ice bag |
| background | S100A8/S100A9 HeterodimerS100A8 (also MRP8 and calgranulin A) is a 10 kDa member of the S100 family, EF-hand superfamily of Ca-binding proteins. It is produced by neutrophils and monocytes, and forms Ca2+-dependent heterodimer/heterotetramer complexes (termed calprotectin) with S100A9. It functions both intracellularly and extracellularly, where it binds to RAGE and CD36. Human S100A8 is 93 amino acids (aa) in length. It contains two EF-hand motifs (aa 12 - 47 and 46 - 81) and one high-affinity Ca2+-binding site (aa 59 - 70). There may be one splice form that shows a 15 aa substitution for the C-terminal 14 amino acids. Although mouse S100A8 is cleaved by MMP-2 after Asn21, it is unclear if human S100A8 is susceptible. Full-length human S100A8 is 57% and 74% aa identical to mouse and canine S100A8, respectively. |
Other Clones
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Unconjugated
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