Recombinant S100A4 Monoclonal Antibody (E-AB-81606)
For research use only.
| Verified Samples |
Verified Samples in WB: Hela Verified Samples in IF: Human lung cancer, hela |
| Dilution | WB 1:1000-1:2000, IHC 1:50-1:100 |
| Isotype | IgG |
| Host | Rabbit |
| Reactivity | Human |
| Applications | WB, IHC-P, IF |
| Clonality | Rabbit Monoclonal |
| Immunogen | A synthetic peptide of human S100A4 |
| Abbre | S100A4 |
| Synonyms | 18A2, 42A, CAPL, Calvasculin, FSP1, Fibroblast specific protein, Fibroblast specific protein 1, Fibroblast specific protein 1 (FSP1), Leukemia multidrug resistance associated protein, Malignan, Malignant transformation suppression 1 (MTS1), calcium Placental protein |
| Swissprot | |
| Calculated MW | 12 kDa |
| Observed MW |
12 kDa
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Cytoplasm, Nucleus. |
| Concentration | 300 μg/mL |
| Buffer | 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% stabilizer and 0.05% protective protein. |
| Purification Method | Affinity Purified |
| Research Areas | Cancer, Cardiovascular, Signal Transduction |
| Clone No. | R01-7H1 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended. |
| background | S100A4 is a member of the S100 family of calcium-binding proteins. The S100 family members have been involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100A4 is known to localize to and function in the nucleus, cytoplasm of cells and the extracellular space. S100A4 has also been shown to be associated with tumor growth, motility, invasion, metastasis, angiogenesis, apoptosis and chemoresistance. It is a fibroblast-specific protein associated with mesenchymal cell morphology and motility, is expressed during epithelial-mesenchymal transformations (EMT) in vivo. It is an improved marker for lung fibroblasts that could be useful for investigating the pathogenesis of pulmonary fibrosis. Overexpression of S100A4 is correlated with a worse prognosis inpatients with various types of cancer. |
Other Clones
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Other Formats
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Unconjugated
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