Recombinant RKIP Monoclonal Antibody (E-AB-81484)
For research use only.
| Verified Samples |
Verified Samples in WB: Rat Rat Brain, C6, 3T3, Hela Verified Samples in IHC: Human tonsil |
| Dilution | WB 1:1000-1:2000, IHC 1:50-1:100 |
| Isotype | IgG |
| Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC-P |
| Clonality | Rabbit Monoclonal |
| Immunogen | A synthetic peptide of human RKIP |
| Abbre | RKIP |
| Synonyms | Epididymis luminal protein 210, Epididymis secretory protein Li 34, Epididymis secretory protein Li 96, HCNP, HCNPpp, HEL 210, HEL S 34, HEL S 96, Hippocampal cholinergic neurostimulating peptide, Neuropolypeptide h3, PBP, PEBP, PEBP 1, PEBP-1, PEBP1, Pebp1, Phosphatidyl |
| Swissprot | |
| Calculated MW | 40 kDa |
| Observed MW |
40 kDa
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Cytoplasm. |
| Concentration | 300 μg/mL |
| Buffer | 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% stabilizer and 0.05% protective protein. |
| Purification Method | Affinity Purified |
| Research Areas | Cancer, Cell Biology, Epigenetics and Nuclear Signaling, Neuroscience |
| Clone No. | R09-4C5 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended. |
| background | Raf kinase inhibitor protein (RKIP),also termed phosphatidylethanolamine binding protein PEBP1,was initially identified as a potent inhibitor of Raf-1/MEK/ERK,NF-kB,and G-protein-coupled receptor signaling pathways. Later RKIP has been further identified as a metastasis suppressor and its loss of expression has been reported in various cancers. Its expression has been proposed as a prognostic marker for patients diagnosed with the above cancers. |
Other Clones
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Other Formats
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Unconjugated
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