Recombinant RENT1 Monoclonal Antibody (E-AB-81603)
For research use only.
| Verified Samples |
Verified Samples in WB: Jurkat, Hela Verified Samples in IF: Human Cholangiocarcinoma, Hela |
| Dilution | WB 1:2000-1:3000, IHC 1:50-1:100, IF 1:50-1:100 |
| Isotype | IgG |
| Host | Rabbit |
| Reactivity | Human |
| Applications | WB, IHC-P, IF |
| Clonality | Rabbit Monoclonal |
| Immunogen | A synthetic peptide of human RENT1 |
| Abbre | RENT1 |
| Synonyms | ATP dependent helicase RENT1, ATP-dependent helicase RENT1, Delta helicase, FLJ43809, FLJ46894, HUPF 1, KIAA0221, NORF 1, NORF1, Nonsense mRNA reducing factor 1, RENT 1, RENT1, Regulator of nonsense transcripts 1, Smg 2, Smg 2 homolog nonsens, hUpf1, pNORF 1, pNORF1 |
| Swissprot | |
| Calculated MW | 124 kDa |
| Observed MW |
124 kDa
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Cytoplasm. Cytoplasm>P-body. Hyperphosphorylated form is targeted to the P-body, while unphosphorylated protein is distributed throughout the cytoplasm. |
| Concentration | 300 μg/mL |
| Buffer | 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% stabilizer and 0.05% protective protein. |
| Purification Method | Affinity Purified |
| Research Areas | Cancer, Epigenetics and Nuclear Signaling |
| Clone No. | R02-2E6 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended. |
| background | This gene encodes a protein that is part of a post-splicing multiprotein complex involved in both mRNA nuclear export and mRNA surveillance.mRNA surveillance detects exported mRNAs with truncated open reading frames and initiates nonsense-mediated mRNA decay (NMD).When translation ends upstream from the last exon-exon junction, this triggers NMD to degrade mRNAs containing premature stop codons.This protein is located only in the cytoplasm.When translation ends, it interacts with the protein that is a functional homolog of yeast Upf2p to trigger mRNA decapping.Use of multiple polyadenylation sites has been noted for this gene. |
Other Clones
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Other Formats
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Unconjugated
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