Recombinant Rat NT-proBNP protein (GST Tag) (PDER100173)
For research use only.
| Synonyms | ANFB_RAT, Brain natriuretic factor prohormone, Gamma-brain natriuretic peptide, Iso-ANP, Natriuretic peptides B, Nppb, preproBNP, proBNP |
| Species | Rat |
| Expression Host | E.coli |
| Sequence | His27-Arg76 |
| Accession | P13205 |
| Calculated Molecular Weight | 30.4 kDa |
| Observed Molecular Weight | 30 kDa |
| Tag | N-GST |
| Bio-activity | Not validated for activity |
| Purity | > 95% as determined by reducing SDS-PAGE. |
| Endotoxin | < 10 EU/mg of the protein as determined by the LAL method |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol. |
| Reconstitution | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis. |
| Background | Cardiac hormone that plays a key role in mediating cardio-renal homeostasis. May also function as a paracrine antifibrotic factor in the heart. Acts by specifically binding and stimulating NPR1 to produce cGMP, which in turn activates effector proteins that drive various biological responses. Likely involved in regulating the extracellular fluid volume and maintaining the fluid-electrolyte balance through natriuresis, diuresis, kaluresis and chloruresis |
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