Recombinant Rat Chymase protein (His Tag) (PDER100207)

For research use only.
Synonyms | Alpha-chymase, CMA1, CYH, CYM, Chymase 1, MCP3P, MCT1, MMCP-5, Mast Cell Chymase, Mcp-5, Mcpt5 |
Species | Rat |
Expression Host | E.coli |
Sequence | Lys81-Pro206 |
Accession | P50339 |
Calculated Molecular Weight | 13.8 kDa |
Observed Molecular Weight | 15 kDa |
Tag | N-His |
Bio-activity | Not validated for activity |
Purity | > 95% as determined by reducing SDS-PAGE. |
Endotoxin | < 10 EU/mg of the protein as determined by the LAL method |
Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol. |
Reconstitution | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis. |
Background | Major secreted protease of mast cells with suspected roles in vasoactive peptide generation, extracellular matrix degradation, and regulation of gland secretion. |
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