Recombinant Rat CD109 protein (His Tag) (PDER100228)
For research use only.
| Synonyms | CD109, CD109 molecule |
| Species | Rat |
| Expression Host | E.coli |
| Sequence | Ser1141-Thr1420 |
| Accession | D4A447 |
| Calculated Molecular Weight | 30.7 kDa |
| Observed Molecular Weight | 35 kDa |
| Tag | N-His |
| Bio-activity | Not validated for activity |
| Form | Lyophilized powder |
| Purity | > 95% as determined by reducing SDS-PAGE. |
| Endotoxin | < 10 EU/mg of the protein as determined by the LAL method |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol. |
| Reconstitution | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis. |
| Background | CD109 is a GPI-anchored member of the alpha-2-macroglobulin (A2M) and complement family of proteins.Cleavage of A2M family proteins within the bait region activates the thioester bond to promote covalent bonding to nucleophilic groups in adjacent molecules.It shares 27-33% aa sequence identity with A2M and complement factors C3, C4, and C5. Alternate splicing of human CD109 generates two isoforms with short deletions and one that is truncated within the bait region. CD109 is expressed on activated T cells and platelets, hematopoietic stem cells, megakaryocyte precursors, vascular endothelial cells, basal and myoepithelial cells of secretory glands, and squamous cell carcinomas. |
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