Recombinant Rat CCL2/JE/MCP-1 Protein(Trx Tag) (PDER100141)
For research use only.
| Synonyms | CCL, Scya, C-C motif chemokine, Small-inducible cytokine A, Monocyte chemotactic protein, Monocyte chemoattractant protein, GDCF, HSMCR, GDCF-2, HC11, HSMCR30, MCAF, MCP-1, Monocyte chemotactic protein 1, SMC-CF, Ccl2, C-C motif chemokine 2, Small-inducible cytokine A2, Monocyte chemotactic protein 1 (MCP-1), Monocyte chemoattractant protein 1, Immediate-early serum-responsive protein JE, Je, Mcp1, Scya2 |
| Species | Rat |
| Expression Host | E.coli |
| Sequence | Gln24-Asn148 |
| Accession | P14844 |
| Calculated Molecular Weight | 33.64 kDa |
| Observed Molecular Weight | 30 kDa |
| Tag | N-Trx |
| Bio-activity | Not validated for activity |
| Purity | > 90% as determined by reducing SDS-PAGE. |
| Endotoxin | < 10 EU/mg of the protein as determined by the LAL method |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol. |
| Reconstitution | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis. |
| Background | Monocyte chemoattractant protein 1 (CCL2/JE/MCP-1), also called CCL2, belongs to a group of CC chemokines located in chromosome 17q11.2. CCL2/JE/MCP-1 protein interacts with chemokine C-C motif receptor 2 (CCR2) to activate and recruit monocytes, macrophages, CD4+ T cells and immature dendritic cells to the site of infection. The presence of CCL2/JE/MCP-1 protein in an adequate concentration is important for granuloma formation and M. tuberculosis clearance. |
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